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酶联免疫吸附试验 (以下简称ELISA) :是酶免疫测定技术中应用最广的技术。 其基本方法是将已知的抗原或抗体吸附在固相载体 ( 聚苯乙烯微量反应板 ) 表面,使酶标记的抗原抗体反应在固相表面进行,用洗涤法将液相中的游离成分洗除。 本文提供 4 種常用的 ELISA 協定:三明治式 ELISA 協定、磷酸化分析步驟、EIA 分析步驟及細胞式分析步驟。 Elisa has been used as a diagnostic tool in medicine, plant pathology, and biotechnology, as well as a quality control check in various industries

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In the most simple form of an elisa, antigens from the sample to be tested are attached to a surface. 酶联免疫吸附实验 (Enzyme linked immunosorbent assay,ELISA)是将 抗原 或 抗体 结合在 固相载体 表面,利用抗原抗体的特异性结合以及抗体或者抗原上标记的酶催化特定底物发生 显色反应,实现目标物检测的免疫分析方法,可测至皮摩尔(pmol)级别。 该测定依赖于抗原-抗体相互作用的原理,并利用酶和比色检测来量化目标分子,主要用于检测和量化生物样品中特定蛋白质、肽、抗体或抗原的存在。 ELISA测定通常应用于各个领域,包括临床诊断、药物研究和生命科学基础研究。

在 ELISA 中,将具有特定结合特性的液体样品添加到反应室或微孔板内的固定固相上。 然后,依次孵育不同的液体试剂,从而在最终液体中产生一些光学变化 (例如,通过酶促反应产物的颜色变化)。

ELISA 是酶联接免疫吸附剂测定 (Enzyme-Linked Immunosorbnent Assay) 的简称。 它是继免疫荧光和放射免疫技术之后发展起来的一种免疫酶技术。 鉴于抗体试剂的特异性以及检测的简易性,ELISA 通常被视为生物样品定量检测的金标准。 在生物医学/生物化学领域,使用抗体-抗原结合连同信号放大的 ELISA 原理的检测非常普遍。 在研究实验室,这些检测可用来定量蛋白水平或通路激活。 Learn about the different methods for performing an elisa assay for protein quantitation, including assay design strategies and reagents.

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